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- Stereotaxic surgery (allows precise microinjection and sampling in brain regions)
- Micro dissection
- Microdialysis
- Seizure activity (vertebrates and invertebrates)
- Behavioral assessment
- Locomotor activity and behavioral sensitization (for abuse potential)
- Conditioned place preference (for abuse potential)
- Analgesia and tolerance
- Anxiety
- Depression
- Thermoregulation
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